9GSQ
DNA binding domain of J-DNA Binding Protein 3 (JBP3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.966 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.701, 40.937, 100.595 |
Unit cell angles | 90.00, 123.82, 90.00 |
Refinement procedure
Resolution | 83.576 - 1.700 |
Rwork | 0.174 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.164 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.9) |
Phasing software | MOLREP (11.9.02) |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 83.576 | 83.580 | 1.730 |
High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
Rmeas | 0.050 | 0.024 | 0.630 |
Number of reflections | 41694 | 304 | 2186 |
<I/σ(I)> | 11.1 | 28.8 | 0.75 |
Completeness [%] | 99.0 | 93 | |
Redundancy | 2.9 | 3 | 2.9 |
CC(1/2) | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 0.2 M potassium sodium tartrate tetrahydrate, 20% w/v polyethylane glycol 3,350 |