9GRH
Crystal structure of the C-terminal phosphatase domain from Saccharomyces cerevisiae Vip1 (apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999993 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.473, 194.857, 84.441 |
| Unit cell angles | 90.00, 112.16, 90.00 |
Refinement procedure
| Resolution | 48.710 - 3.200 |
| R-factor | 0.2249 |
| Rwork | 0.218 |
| R-free | 0.27600 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.483 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.710 | 3.230 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmeas | 0.153 | 1.714 |
| Number of reflections | 48030 | 7738 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 1.000 | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M Tris pH 8.0 |
