9GR9
Homodimer of BACH1 BTB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91847 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.471, 51.679, 106.413 |
| Unit cell angles | 90.00, 94.57, 90.00 |
Refinement procedure
| Resolution | 53.040 - 2.250 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.385 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.040 | 2.480 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 18483 | 924 |
| <I/σ(I)> | 5.2 | 1.4 |
| Completeness [%] | 91.3 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.996 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.1 M Tris-HCL, 0.2 M Li2SO4, 30 % (w/v) PEG4000 |
