9GQU

Crystal structure of NtcA from S. elongatus in apo form A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-06
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9797
Spacegroup name	P 21 21 21
Unit cell lengths	66.502, 88.845, 99.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.780 - 2.850
R-factor	0.2152
Rwork	0.213
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.131
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.300	3.000
High resolution limit [Å]	2.850	2.850
Number of reflections	14209	14209
<I/σ(I)>	12.1
Completeness [%]	99.0
Redundancy	4.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	NtcA protein was at 6,1 mg/ml IN 50 mM sodium citrate pH 6.5, 0.5 M NaCl, 5 mM magnesium cloride, 50 mM arginine hydrocloride, 50 mM Na L-glutamate. CRYSTALLIZATION SOLUTION: 0.1M Na HEPES pH 6.5, 20,5% PEG 4000. CRYO PROTECTANT: 25% PEG 400