9GPH

Teth514_1788 1,2-beta-oligomannan phosphorylase in complex with mannose (-1) and phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03987
Spacegroup name	P 21 21 21
Unit cell lengths	67.386, 67.570, 299.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.005 - 1.700
Rwork	0.192
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.521
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.005	1.790
High resolution limit [Å]	1.700	1.700
Number of reflections	150586	20773
<I/σ(I)>	2
Completeness [%]	99.5
Redundancy	3.6
CC(1/2)	0.971	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	to be retrieved