9GPB
THE ALLOSTERIC TRANSITION OF GLYCOGEN PHOSPHORYLASE
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.000, 190.000, 88.200 |
| Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.900 |
| Rwork | 0.177 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 4.000 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.900 * |
| Rmerge | 0.120 * |
| Total number of observations | 86341 * |
| Number of reflections | 59294 * |
| Completeness [%] | 81.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7 * | 16 * | took 18 from original paper * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | buttom | protein | 7 (mg/ml) | |
| 2 | 1 | buttom | AMP | 0.01 (M) | |
| 3 | 1 | buttom | magnesium acetate | 0.05 (M) | |
| 4 | 1 | buttom | ammonium sulfate | 0.25 (%sat) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.32 (%sat) | |
| 6 | 1 | reservoir | AMP | 0.01 (M) | |
| 7 | 1 | reservoir | magnesium acetate | 0.05 (M) |
