9GO2

C1C2 Channelrhodopsin - SMX Light activated structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-07-07
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	61.400, 141.700, 94.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.850 - 2.700
R-factor	0.2399
Rwork	0.237
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.810
Data reduction software	CrystFEL (0.10.2)
Data scaling software	CrystFEL (0.10.2)
Phasing software	PHENIX (1.20_4459)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.850	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	7315	72
<I/σ(I)>	9.98	1.16
Completeness [%]	62.6
Redundancy	348.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	100 mM sodium citrate (pH 6.0), 30% PEG500DME, 100 mM MgCl2, 100 mM NaCl, and 100 mM (NH4)2SO4.