9GNS
X-ray structure of Human holo aromatic L-amino acid decarboxylase (AADC) complex with Carbidopa at physiological pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9198 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 106.921, 106.921, 219.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.300 - 1.930 |
| R-factor | 0.1752 |
| Rwork | 0.174 |
| R-free | 0.19240 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.809 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | FFT |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.600 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.047 | 0.509 |
| Number of reflections | 56043 | 3731 |
| <I/σ(I)> | 13.9 | 2.3 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 0.1 M Hepes, 46% PEG 200 |
