9GNG
mouse VDAC1 in complex with MPD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-20 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.775 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.426, 58.354, 66.822 |
| Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
| Resolution | 66.140 - 2.400 |
| R-factor | 0.2452 |
| Rwork | 0.243 |
| R-free | 0.29850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.684 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.140 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.164 | 8.492 |
| Number of reflections | 15147 | 1525 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.5 | 95.9 |
| Redundancy | 12.3 | 8.8 |
| CC(1/2) | 0.998 | 0.142 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12-18% MPD, 0.1 M Tris-HCl, 10% PEG400, pH 8.5 |
