9GNF
A novel aminotransferase from Streptomyces sp. Gabaculine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 113.15 |
| Detector technology | PIXEL |
| Collection date | 2022-04-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.463, 177.253, 112.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.585 - 1.310 |
| Rwork | 0.165 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.014 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.77)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.590 | 1.344 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Number of reflections | 237241 | 15399 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.3 | 92.3 |
| Redundancy | 5 | 13.3 |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 292.15 | 0.2 M Potassium thiocynate 0.1 M Bis_tris propane 6.5 20% w/v PEG 3350 1 mM PLP |
