9GLW
NRas-Q61R-GTP in complex with the peptide MPB2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99997 |
| Spacegroup name | H 3 |
| Unit cell lengths | 112.220, 112.220, 58.584 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.110 - 2.100 |
| R-factor | 0.1993 |
| Rwork | 0.197 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RIZL |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.110 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.091 | 0.838 |
| Number of reflections | 16013 | 401 |
| <I/σ(I)> | 9.96 | 1.89 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 4.92 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 30% PEG 1000, 0.2 M Na2HPO4 |
