9GLU
Crystal structure of KRasG12D-GDP in complex with the peptide MPB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.407, 79.440, 128.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.960 - 1.900 |
| R-factor | 0.1851 |
| Rwork | 0.184 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1VTYV |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.260 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.707 |
| Number of reflections | 34233 | 685 |
| <I/σ(I)> | 13.57 | 2.86 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.45 | 6.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.1 mM BisTris pH 5.5 |
