9GLS

Crystal Structure of Human UBCH5B C85E

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	298
Detector technology	PIXEL
Collection date	2024-02-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 21
Unit cell lengths	47.916, 49.401, 63.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.928 - 1.250
Rwork	0.189
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.842
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.930	1.295
High resolution limit [Å]	1.250	1.250
Rmeas	0.047	0.945
Number of reflections	41718	4119
<I/σ(I)>	12.33	1.35
Completeness [%]	98.8	98.59
Redundancy	3.7	3.8
CC(1/2)	0.998	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	10% PEG 200, 0.1 M Bis-Tris propane, pH 9, 15% PEG 8000