9GKZ

Crystal Structure of Acetylpolyamine amidohydrolase (ApaH) from Pseudomonas sp. M30-35

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979510
Spacegroup name	P 21 21 2
Unit cell lengths	147.342, 48.858, 82.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.670 - 2.720
R-factor	0.1595
Rwork	0.156
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.927
Data reduction software	XDS (Jan 10, 2022 (BUILT 20220220))
Data scaling software	XDS (Jan 10, 2022 (BUILT 20220220))
Phasing software	PHASER (v3.60.0)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.671	2.763
High resolution limit [Å]	2.716	2.716
Rmerge	0.237	1.024
Rmeas	0.251	1.092
Rpim	0.081	0.372
Number of reflections	16673	795
<I/σ(I)>	8.9	2.3
Completeness [%]	100.0	100
Redundancy	9.3	8.6
CC(1/2)	0.988	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1M sodium acetate pH 4.5 20% (w/v) PEG3000