9GKK
BetP heterotrimeric complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | JEOL CRYO ARM 200 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.04 |
| Number of Particle | 1509120 |
| Software | CTFFIND,UCSF Chimera,REFMAC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
