9GJ4

Structure of the amyloid-forming peptide LYNleQNY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-22
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 21 21 21
Unit cell lengths	4.826, 20.872, 42.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.750 - 1.550
R-factor	0.1377
Rwork	0.136
R-free	0.15430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.915
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.750	1.610
High resolution limit [Å]	1.550	1.550
Rmeas	0.185	0.635
Number of reflections	786	91
<I/σ(I)>	5.4	1.5
Completeness [%]	100.0
Redundancy	5.63
CC(1/2)	0.992	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		277	10 v/v% ethanol, 277K