9GFQ

Structure of PRD1 SSB P12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000030
Spacegroup name	P 21 21 21
Unit cell lengths	36.434, 50.999, 56.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.800 - 1.700
Rwork	0.228
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.800	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	12325	595
<I/σ(I)>	9.4
Completeness [%]	99.0	95
Redundancy	12.4
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	From Morpheus[1], Molecular Dimensions: 0.12 M monosaccharides (0.2M D-glucose; 0.2M D-mannose; 0.2M D-galactose; 0.2M L-fucose; 0.2M D-xylose; 0.2M N-acetyl-D-glucosamine), 0.1 M buffer system 3 (Tris [base]; BICINE) pH 8.5, and 37.5 % v/v precipitant mix 4 (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350)