9GFF
BCR Fab from the subset 1 chronic lymphocytic leukaemia case P10015
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 63 |
| Unit cell lengths | 99.952, 99.952, 123.048 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 86.560 - 2.800 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.695 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.570 | 2.640 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rpim | 0.043 | 0.746 |
| Number of reflections | 23294 | 2319 |
| <I/σ(I)> | 14 | 1.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 12.4 | 11.7 |
| CC(1/2) | 0.999 | 0.465 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M ammonium succinate pH 7 and 1.5 M ammonium sulfate |
