9GEZ
Crystal structure of thioredoxin reductase from Cryptosporidium parvum in the "waiting" position
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.553, 74.177, 188.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.790 - 2.880 |
| R-factor | 0.23297 |
| Rwork | 0.231 |
| R-free | 0.26393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.637 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.790 | 3.000 |
| High resolution limit [Å] | 2.880 | 2.900 |
| Rmerge | 0.500 | 1.830 |
| Number of reflections | 23509 | 2295 |
| <I/σ(I)> | 7.56 | |
| Completeness [%] | 96.3 | |
| Redundancy | 12.1 | |
| CC(1/2) | 0.980 | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294.15 | 0.1 M Tris/HCl, 0.1 M KSCN, PEG 2000 MME |
