9GES
Crystal structure of HEI10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-03 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 1.27806 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.198, 73.857, 124.032 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 43.460 - 2.430 |
| R-factor | 0.2521 |
| Rwork | 0.250 |
| R-free | 0.28940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.456 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.238 | 2.781 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.115 | 1.201 |
| Rpim | 0.051 | 0.479 |
| Number of reflections | 11283 | 565 |
| <I/σ(I)> | 9.4 | 1.7 |
| Completeness [%] | 31.3 | 4.7 |
| Redundancy | 6.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES sodium salt pH 7.5 30% (w/v) MPD; 5% (w/v) PEG 4000 |
