9GDK
Jumonji domain-containing protein 1C with crystallization epitope mutations L2440Y:G2444H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.200, 98.250, 87.400 |
| Unit cell angles | 90.00, 92.04, 90.00 |
Refinement procedure
| Resolution | 31.369 - 1.780 |
| Rwork | 0.209 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.859 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.77)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.370 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.086 | 0.936 |
| Number of reflections | 71577 | 18899 |
| <I/σ(I)> | 12 | 1.7 |
| Completeness [%] | 98.1 | 99.4 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | LFS well F04 20% PEG3350 10% ethylene glycol 0.1M bis-tris-propane pH 6.5 0.2M potassium thiocyanate |
