9GDB
RNAP-TopoI complex on bubble scaffold - consensus reconstruction
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.8 |
| Software | Warp,PHENIX,Coot,cryoSPARC,SerialEM,UCSF Chimera,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
