9GCC
CRYSTAL STRUCTURE OF HUMAN CHYMASE IN COMPLEX WITH COMPOUND47
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541870 |
| Spacegroup name | P 43 |
| Unit cell lengths | 74.150, 74.150, 49.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.140 - 1.793 |
| Rwork | 0.164 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.745 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.140 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.066 | 0.280 |
| Number of reflections | 25367 | 3649 |
| <I/σ(I)> | 29.7 | 8.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.01M ZnCl2, 0.1M TRIS at pH 8 and 20% PEG 6000 |
