9GAW

High-resolution structure of the Anaphase-promoting complex/cyclosome (APC/C) bound to co-activator Cdh1

Replaces:  8S4GReplaces:  7QE7

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK III

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	FEI FALCON III (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.9
Number of Particle	1800000
Software	cryoSPARC,Coot,RELION,EPU,UCSF Chimera,PHENIX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION