9GA1
Structure of Pentameric Outer Membrane Protein A from Bdellovibrio bacteriovorus
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 175.430, 191.346, 184.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.540 - 2.800 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.23910 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.983 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | CRANK2 |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.540 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.161 | 1.011 |
| Rmeas | 0.168 | 1.050 |
| Rpim | 0.045 | 0.280 |
| Number of reflections | 76317 | 7548 |
| <I/σ(I)> | 12.26 | 1.48 |
| Completeness [%] | 99.9 | 99.96 |
| Redundancy | 13.9 | |
| CC(1/2) | 0.998 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M NaCl, 25% v/v pentaerythritol propoxylate (5/4 PO/OH), 10% v/v DMSO |
