9G8V

StmPr1, Stenotrophomonas maltophilia Protease 1, 36 kDa alkine serine protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.968
Spacegroup name	C 2 2 21
Unit cell lengths	60.385, 86.382, 131.573
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.541 - 1.637
Rwork	0.173
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.869
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.541	1.665
High resolution limit [Å]	1.637	1.637
Rmerge	0.178	1.322
Rmeas	0.185
Rpim	0.050	0.377
Number of reflections	42779	2107
<I/σ(I)>	1.38
Completeness [%]	100.0	100
Redundancy	13.4	13.2
CC(1/2)	0.997	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	1.8 M ammonium sulfate 0.1 M Tris