9G8H

Crystal structure of a galactose oxidase in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-21
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	48.005, 61.406, 186.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.390 - 2.100
R-factor	0.16453
Rwork	0.161
R-free	0.23667
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.420
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.390	2.320
High resolution limit [Å]	2.090	2.090
Number of reflections	22173	1109
<I/σ(I)>	5.6
Completeness [%]	89.2
Redundancy	5.1
CC(1/2)	0.988	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium HEPES and MOPS pH 7.5, 0.03 M magnesium chloride hexahydrate, 0.03 M calcium chloride dihydrate, 12.5% (v/v) MPD, 12.5% (w/v) PEG 1000 and 12.5% (w/v) PEG 3350