9G7W

CTX-M-14 apo serial crystallography temperature series; 20C, 293K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2024-02-26
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.976
Spacegroup name	P 32 2 1
Unit cell lengths	41.850, 41.850, 232.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	77.620 - 1.700
R-factor	0.169
Rwork	0.168
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.675
Data reduction software	CrystFEL (0.10.2)
Data scaling software	CrystFEL (0.10.2)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.620	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	27455	2685
<I/σ(I)>	7.4	2.17
Completeness [%]	100.0
Redundancy	353.9
CC(1/2)	0.979	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	CTX-M-14 solution (22 mg/ml) was mixed with 45% precipitant solution (40% PEG8000, 200mM lithium sulfate, 100mM sodium acetate, pH 4.5) and with 5% undiluted seed stock in batch crystallization setups