9G7O
Crystal structure of Janthinobacterium lividum PE-like toxin, Jlx
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-01 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 224.625, 143.241, 110.670 |
| Unit cell angles | 90.00, 119.35, 90.00 |
Refinement procedure
| Resolution | 115.600 - 1.750 |
| Rwork | 0.213 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.353 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.600 | 1.950 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 2.070 | |
| Rmeas | 0.103 | 2.150 |
| Rpim | 0.028 | 0.558 |
| Number of reflections | 186952 | 9348 |
| <I/σ(I)> | 12.5 | 1.2 |
| Completeness [%] | 94.6 | 71.3 |
| Redundancy | 14.1 | 14.7 |
| CC(1/2) | 1.000 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294.16 | 0.1 M Amino acids, 0.1 M Buffer System 1 pH 6.5, 30 % v/v Precipitant Mix 1 (condition H1 of the Morpheus Screen, Molecular Dimensions) |
