9G7D

Crystal structure of ASGPR with bound IMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	113.775, 32.689, 40.819
Unit cell angles	90.00, 91.98, 90.00

Refinement procedure

Resolution	56.850 - 1.588
R-factor	0.1848
Rwork	0.182
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.840
Data reduction software	autoPROC
Data scaling software	STARANISO ((Version 1.1.7))
Phasing software	BUSTER
Refinement software	BUSTER (2.11.8 (20-OCT-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.854	1.862
High resolution limit [Å]	1.588	1.588
Rmerge	0.117	0.744
Number of reflections	11473	575
<I/σ(I)>	7.4
Completeness [%]	55.7	8.2
Redundancy	3.3	3.2
CC(1/2)	0.995	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PROTEIN BUFFER: 20 MM TRIS-HCL (PH 7.4), 25 MM CACL2. RESERVOIR: 100 MM TRIS-HCL (PH 7.5), 28% PEG4000, PH 7.50, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K