9G5L

Stapylococcus aureus MazF in complex with nanobody 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 41 21 2
Unit cell lengths	99.040, 99.040, 70.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.600 - 1.822
R-factor	0.202336255612
Rwork	0.201
R-free	0.22814
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.374
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	1.890
High resolution limit [Å]	1.819	1.819
Number of reflections	31690	3391
<I/σ(I)>	20.27
Completeness [%]	99.8	99
Redundancy	13.2
CC(1/2)	1.000	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M TRIS-HCl pH 8.5, 8% (w/v) PEG-8000