9G58

Pseudomonas fluorescens periplasmic aspartic peptidase Pfl01_1762 (RloA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41
Unit cell lengths	90.042, 90.042, 37.166
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.020 - 2.400
R-factor	0.2147
Rwork	0.211
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.020	2.360
High resolution limit [Å]	2.280	2.280
Rmerge	0.097	1.069
Number of reflections	26253	2402
<I/σ(I)>	8.91	1.42
Completeness [%]	98.9	91.98
Redundancy	5.7	5.4
CC(1/2)	0.994	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M ammonium acetate, 20% PEG 3350 and 0.1 M HEPES pH 8.0 (soak with condition incl. 20% xylitol)