9G4G

Staphylococcus aureus MazF in complex with Nanobody 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	49.090, 133.750, 100.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.084 - 2.896
R-factor	0.255352559346
Rwork	0.253
R-free	0.30320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.084	3.070
High resolution limit [Å]	2.896	2.896
Number of reflections	7201	1195
<I/σ(I)>	9.51	2.65
Completeness [%]	93.5
Redundancy	4.8
CC(1/2)	0.990	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M ammoniumsulphate, 0.1 M Na-cacodylate pH 6.5, 30% (w/v) PEG-8000