9G4D
Cryo-EM reconstruction of the full-length H209N mutant E. coli transmembrane formate transporter FocA
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.97 |
| Number of Particle | 1150907 |
| Software | cryoSPARC,PHENIX,EPU,UCSF ChimeraX,Coot |
| CTF correction | NONE |
