9G48

Staphylococcus aureus MazF in complex with Nanobody 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 31 2 1
Unit cell lengths	60.140, 60.140, 246.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.761 - 2.144
R-factor	0.217533490853
Rwork	0.216
R-free	0.24538
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.540
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.761	2.280
High resolution limit [Å]	2.144	2.144
Number of reflections	28443	4525
<I/σ(I)>	6.6
Completeness [%]	96.8	82.9
Redundancy	2.845
CC(1/2)	0.995	0.463

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M CdCl2, 0.1 M Na-acetate pH 4.6, 30% (w/v) PEG-400