9G2G
Staphylococcus aureus MazF in complex with nanobody 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.600, 50.873, 52.083 |
| Unit cell angles | 67.50, 79.80, 85.35 |
Refinement procedure
| Resolution | 46.996 - 1.800 |
| R-factor | 0.197811004035 |
| Rwork | 0.195 |
| R-free | 0.24385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.745 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.996 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 34576 | 2227 |
| <I/σ(I)> | 13.55 | 1.78 |
| Completeness [%] | 78.8 | 34.4 |
| Redundancy | 2.65 | |
| CC(1/2) | 0.998 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.2 M MgCl2, 0.1 M TRIS pH 8.5, 30% (w/v) PEG4000 |
