9G2B
Yeast RNA polymerase I elongation complex stalled by an apurinic site, 12-subunit
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Software | PHENIX,RELION,cryoSPARC,crYOLO,CTFFIND,Gctf,EPU,UCSF Chimera,UCSF ChimeraX |
| CTF correction | PHASE FLIPPING ONLY |
