9G11
sugar/H+ symporter STP6 in inward occluded conformation with glucose bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96863 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 122.221, 122.221, 139.091 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 84.230 - 3.200 |
R-factor | 0.2303 |
Rwork | 0.227 |
R-free | 0.25160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.858 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.230 | 3.420 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.226 | 1.168 |
Rmeas | 0.258 | 1.330 |
Rpim | 0.123 | 0.627 |
Total number of observations | 80631 | 15369 |
Number of reflections | 19456 | 3591 |
<I/σ(I)> | 7.3 | 1.5 |
Completeness [%] | 96.5 | |
Redundancy | 4.1 | 4.3 |
CC(1/2) | 0.981 | 0.347 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292.15 | 30% PEG smear (PEG2000, PEG3000, PEG3500, PEG4000, PEG5000), 0.1M Hepes pH 8.0, 0.4M NaCl |