9G0S
Xenopus tropicalis undecorated microtubule - 14 protofilament, 3-start helix
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 192000 |
| Software | crYOLO,RELION,PHENIX,EPU,CTFFIND,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
