9G0D

Structure of human Mical1 bMERB_V978A domain:Rab10 complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.873130
Spacegroup name	P 1 21 1
Unit cell lengths	53.770, 49.650, 79.330
Unit cell angles	90.00, 98.54, 90.00

Refinement procedure

Resolution	36.290 - 2.050
R-factor	0.2032
Rwork	0.201
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.808
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1-3660_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.290	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.095	1.338
Rmeas	0.104	1.442
Number of reflections	26092	1923
<I/σ(I)>	11.36	1.8
Completeness [%]	99.3	99.7
Redundancy	6.78
CC(1/2)	0.997	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Imidazole pH 8.0, 5% (w/v) PEG 3000 and 30% (v/v) PEG 200