9G0C

Structure of human Mical1 bMERB_V978A_V985A domain:Rab10 complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999968
Spacegroup name	P 1 21 1
Unit cell lengths	53.390, 48.560, 78.770
Unit cell angles	90.00, 97.98, 90.00

Refinement procedure

Resolution	41.220 - 1.800
R-factor	0.1946
Rwork	0.193
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.828
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.220	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.051	0.727
Rmeas	0.055	0.791
Number of reflections	37165	2758
<I/σ(I)>	17.93	1.85
Completeness [%]	99.5
Redundancy	6.9
CC(1/2)	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.17 M Sodium acetate, 0.085 M Tris-HCl pH 8.5, 25.5% (w/v) PEG 4000 and 15% (v/v) glycerol