9G0B
Rhinovirus A2 uncoating intermediate revealing the natural pocket factor (pH 5.8 and 4 degrees Celsius)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Number of Particle | 16372 |
| Software | RELION,Coot,PHENIX,EPU,CTFFIND,UCSF Chimera |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
