9FZC
Structure of OmpA-short in complex with nanobody Nb01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-03 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.048, 55.296, 107.805 |
Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
Resolution | 107.790 - 2.270 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.594 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.790 | 2.314 |
High resolution limit [Å] | 2.270 | 2.275 |
Rmerge | 0.146 | 0.876 |
Number of reflections | 30638 | 1534 |
<I/σ(I)> | 10.7 | 2.1 |
Completeness [%] | 100.0 | |
Redundancy | 6.76 | |
CC(1/2) | 0.997 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M sodium acetate pH 5.5, 0.2 M calcium acetate, 25 % PEG MME 2K |