9FYS

D11 mAbs bound to alpha-Bungarotoxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	79.847, 83.804, 102.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.170 - 1.320
R-factor	0.1685
Rwork	0.167
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.507
Data reduction software	DIALS (v1.0)
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	1.367
High resolution limit [Å]	1.320	1.320
Number of reflections	158875	14966
<I/σ(I)>	8.99
Completeness [%]	97.2
Redundancy	13.4
CC(1/2)	0.988	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES: NaOH, 12 % PEG 20000, pH 6.5