9FYO

Lacto-N-biosidase from Trueperella pyogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.6888
Spacegroup name	P 21 21 21
Unit cell lengths	90.759, 93.686, 94.037
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.300 - 2.770
R-factor	0.2292
Rwork	0.227
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.628
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.300	2.860
High resolution limit [Å]	2.770	2.770
Rmerge	0.083	1.000
Rpim	0.024	0.281
Number of reflections	20739	2036
<I/σ(I)>	20.31
Completeness [%]	98.5
Redundancy	13.4
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294.15	0,01 M Nickel II chloride hexahydrate, 0,1M Tris pH8,5 and 20% PEG 2000 MME