9FYN
Lacto-N-biosidase from Trueperella pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.6888 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.788, 93.628, 93.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.880 - 1.790 |
| R-factor | 0.2073 |
| Rwork | 0.206 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.641 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.880 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.077 | 1.000 |
| Rmeas | 0.080 | 1.000 |
| Rpim | 0.021 | 1.000 |
| Number of reflections | 75789 | 7456 |
| <I/σ(I)> | 16.91 | |
| Completeness [%] | 99.5 | |
| Redundancy | 13.8 | |
| CC(1/2) | 1.000 | 0.261 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294.15 | 0,01 M Nickel II chloride hexahydrate, 0,1M Tris pH8,5 and 20% PEG 2000 MME |
