9FYM

Lacto-N-biosidase from Treponema denticola ATCC 35405

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	43.954, 50.701, 153.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.150 - 1.340
R-factor	0.1702
Rwork	0.169
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.270
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.150	1.390
High resolution limit [Å]	1.340	1.340
Rmerge	0.083	1.000
Rmeas	0.089	1.000
Rpim	0.031	1.000
Number of reflections	77556	7469
<I/σ(I)>	11.31	0.83
Completeness [%]	99.6	97.97
Redundancy	8.2	7.1
CC(1/2)	0.998	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294.15	10% w/v PEG 8000, 9% ethylene glycol, 0.1M HEPES pH 7.5