9FYM
Lacto-N-biosidase from Treponema denticola ATCC 35405
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.954, 50.701, 153.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.150 - 1.340 |
| R-factor | 0.1702 |
| Rwork | 0.169 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.270 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.150 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.083 | 1.000 |
| Rmeas | 0.089 | 1.000 |
| Rpim | 0.031 | 1.000 |
| Number of reflections | 77556 | 7469 |
| <I/σ(I)> | 11.31 | 0.83 |
| Completeness [%] | 99.6 | 97.97 |
| Redundancy | 8.2 | 7.1 |
| CC(1/2) | 0.998 | 0.464 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | 10% w/v PEG 8000, 9% ethylene glycol, 0.1M HEPES pH 7.5 |
