9FXV
Influenza polymerase A C-terminal domain of PA subunit with peptide inhibitor containing two norleucines
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-27 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.857, 119.561, 123.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.910 - 2.300 |
| R-factor | 0.2426 |
| Rwork | 0.242 |
| R-free | 0.27948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.910 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 | 0.833 |
| Rmeas | 0.086 | 1.126 |
| Rpim | 0.044 | 0.752 |
| Total number of observations | 2437 | |
| Number of reflections | 24417 | 1630 |
| <I/σ(I)> | 9.9 | 0.7 |
| Completeness [%] | 95.0 | |
| Redundancy | 3.2 | 1.5 |
| CC(1/2) | 0.997 | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1 M MOPS/HEPES; 15% PEG 3350 |
