9FXT

L2A5 Fab in complex with STn-Ser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.67019
Spacegroup name	P 21 21 21
Unit cell lengths	48.355, 125.277, 190.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.690 - 2.300
R-factor	0.2635
Rwork	0.261
R-free	0.31210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5109: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.690	6.830	2.440
High resolution limit [Å]	2.300	4.860	2.300
Rmerge	0.166
Rmeas	0.170	0.062	1.300
Number of reflections	52514	3137	5942
<I/σ(I)>	15.6
Completeness [%]	99.9
Redundancy	23.27
CC(1/2)	0.998	0.993	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	22% PEG 3350, 0.1M NH4Cl pH7