9FXF

VHH variant adression natural cytotoxicity triggering receptor 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97626
Spacegroup name	C 1 2 1
Unit cell lengths	88.022, 34.951, 46.269
Unit cell angles	90.00, 108.93, 90.00

Refinement procedure

Resolution	24.520 - 1.067
R-factor	0.1934
Rwork	0.193
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (23-JAN-2024))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.522	1.170
High resolution limit [Å]	1.067	1.067
Rmeas	0.076
Rpim	0.028	0.305
Number of reflections	37662	1883
<I/σ(I)>	12.4
Completeness [%]	75.9
Redundancy	6.5
CC(1/2)	0.996	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 M Ammonium phosphate, 0.1 M Sodium citrate pH 5.5